Which test in the IMViC series measures indole production?

The Indole Test is specifically designed to measure the ability of an organism to produce indole from the amino acid tryptophan. The process involves incubating the bacterial culture in a medium that contains tryptophan and then adding a reagent, usually Kovac's reagent, to detect indole production. Upon the addition of Kovac's reagent, the presence of indole is indicated by the development of a red-colored ring at the top of the culture medium if indole is present.

In the context of the IMViC series, each test serves a distinct purpose: the Methyl Red Test assesses acid production from glucose fermentation, the Voges-Proskauer Test detects acetoin production, and the Citrate Test evaluates the ability to utilize citrate as a sole carbon source. None of these tests measure indole production, making the Indole Test the clear focus for this specific function within the IMViC series.