What method can be used to sterilize media that cannot be heated?

Filtration is an effective method for sterilizing media that cannot withstand heat. It utilizes a physical barrier, typically a membrane with pores small enough to trap bacteria and other microorganisms, allowing the liquid to pass through while retaining the contaminants. This is especially useful for heat-sensitive substances, such as certain culture media, antibiotics, or vitamins, which might degrade or lose their efficacy if subjected to high temperatures.

Filtration provides a way to achieve sterility without the risks associated with heat, making it a preferred technique in laboratories where maintaining the integrity of the media is crucial. By using this method, microorganisms can be effectively removed, ensuring that the final product is sterile and suitable for use in various applications.

Other methods, such as chemical disinfectants and antiseptics, may not guarantee the complete removal of all viable microorganisms and are typically not suitable for sterilizing media. Additionally, stating that the media "cannot be sterilized" would be incorrect, as filtration provides a viable alternative in such cases.