Apollon Bacteriology Practice Test

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• Which of the following colony appearances on Levine's EMB agar indicates lactose fermentation?
• What is the preferred medium for the culture of most Haemophilus?
• What type of test is used for serotyping when capsules are present?
• Which of the following mycobacteria are classified as slow growers?
• Which media allows for clear examination of mycobacterial colonies microscopically?
• The time it takes half a dose of antibiotic to disappear from the blood is called?
• Which aerobic, gram-positive, sporulating rods can cause food poisoning?
• What are "lepra" cells?
• Bacteriostatic agents do which of the following?
• Which biochemical test does Listeria monocytogenes produce a positive result in?
• What is the term for the temperature range in which bacteria grow best?
• What are iodophores primarily composed of when combined with iodine?
• What is the primary sugar source in Hektoen Enteric Agar?
• What organism is known to cause pinkeye?
• What role does sodium thiosulfate play in triple sugar iron agar?
• What is required for hydrogen sulfide production beyond using triple sugar iron agar?
• What characteristic differentiates Clostridium perfringens in hemolytic activity?
• What characteristic of the Ziehl-Neelsen method helps determine acid-fastness?
• Which of the following are the best indicators of poor storage?
• Which method is often used for visualizing fungi in clinical specimens?
• What are the characteristics of an organism isolated from a blood culture described as a Gram-negative rod?
• Which diphtheroid can typically be found in the normal throat?
• Nagler agar is a selective medium for which type of bacteria?
• What type of test is used to report results when substituting a biochemical test for a serological test?
• What does the term 'presumptive' indicate in a laboratory report?
• Why should blood agar plates for throat cultures be incubated both aerobically and anaerobically?
• What is the most common cause of bacterial food poisoning in the United States?
• What is the primary advantage of using an autoclave for sterilization?
• Which serological test is done for Chlamydia trachomatis on genital smears?
• What characterizes Holt-Harris EMB medium?
• Staphylococcus aureus can be isolated from stool cultures by using which type of medium?
• Which anaerobic, gram-positive rod is known for producing terminal "lollipop" spores?
• What is the purpose of enrichment fluid media when culturing Enterobacteriaceae?
• Which of the following is the most abundant flora found in throat cultures?
• What color indicates a negative enzymatic hydrolysis result in a Tween 80 test?
• Which of the following organisms is urease-positive?
• Which of the following best describes Gram-negative, coffee bean shaped diplococci with adjacent sides flatted?
• How often should the face velocity of safety cabinets be checked?
• Why should thioglycollate broth be boiled for 10 minutes before use?
• Which of the following is a common feature of both Staphylococcus and Streptococcus species?
• An organism isolated from a urine culture has a Gram-negative rod with specific IMViC results. What is the likely organism?
• Which device is recommended for removing antimicrobials prior to culturing?
• Which filter type can achieve 100% sterility?
• What characterizes "rice water" stools?
• Genital cultures for sexually transmitted diseases include all of the following EXCEPT?
• Which equipment's settings should be reviewed monthly according to best practices?
• Which test would NOT be suitable for identifying Neisseria species?
• What is the typical characteristic of bacteria classified as mesophilic?
• Which of the following have a bacterial type cell wall?
• For quality control when monitoring reagents, how often should reagent disks be checked?
• Which of the following bacteria is anaerobic?
• What is the purpose of checking the rpm settings on centrifuges?
• A non-hemolytic, catalase-positive, coagulase-negative, gram positive coccus is most likely to be which of the following?
• For cultures of beta-hemolytic streptococci, which incubation condition must be included?
• What is the purpose of the ONPG test?
• Which of the following is not a characteristic of bacteriostatic antibiotics?
• Identify the organism associated with meningitis?
• Very large gram-negative rods with spores are seen in nonturbid thioglycollate broth culture of spinal fluid, but there is no growth anaerobically or aerobically. What is the most likely explanation?
• In the Kirby-Bauer susceptibility test, the 0.5 McFarland nephelometer standard is used to?
• Babes-Ernst granules are characteristic of which organism?
• Which of the following is a small, nonmotile, coccobacillus that is cultured in the yolk sac of a chick embryo?
• What is the type of hemolysis described as a small zone of alpha-hemolysis surrounded by a zone of beta-hemolysis after refrigeration?
• Which test in the IMViC series measures indole production?
• What is the role of phenylethyl alcohol in media?
• What color indicates a positive oxidase test?
• Nasopharyngeal swabs are recommended for the detection of carriers of which organisms?
• Which organism serves as normal flora in the respiratory tract?
• In the Quellung test, what structure's antigenic specificity is crucial for the result?
• What is the purpose of the bile esculin test?
• Which organism is identified as a curved, gram-negative rod that is nonfermentive, nitrate-positive, and microaerophilic?
• Mycoplasma pneumoniae is incubated in which condition?
• A fastidious, oxidase-positive, gram-negative coccus from a rectal swab might be?
• A fastidious, small to filamentous, gram-negative rod from nasopharyngeal swab is most likely to be
• Which of the following is NOT an anaerobe?
• Acid-fast bacteria appear what color microscopically?
• Acid-fast bacilli retain which dye?
• What condition is primarily associated with group A streptococci?
• What aspect of laboratory safety is enhanced through frequent temperature logging?
• What is a key physiological trait of psychrophilic bacteria?
• Which of the following statements about citrate-positive organisms is correct?
• What is the morphological classification of Corynebacterium diphtheriae?
• In Brilliant Green Agar, what does the inhibitor target?
• Which staining method is usually used for cerebrospinal fluid smears?
• If a negative nitrate reduction test does not change color after the addition of powdered zinc, how is it reported?
• Which of the following describes PPNG gonococci characteristics?
• Which of the following will differentiate Listeria monocytogenes from the corynebacteria?
• When performing the oxidase test, which technique can be used?
• Which of the following is commonly used to isolate Streptococcus species?
• What will Nocardia grow on?
• What colors are the colonies on Hektoen Enteric Agar?
• What is the best method for the identification of Mycoplasma species?
• What type of treatment is indicated for surfaces that require disinfection but not sterilization?
• When should safety cabinets be checked for their face velocity?
• What is the purpose of potassium tellurite in tellurite medium?
• Which organism is often used as an indicator for testing the effectiveness of steam sterilization?
• What does the size of the zone of growth inhibition correlate with if the correct procedure has been used?
• Which of the following genera are oxidase positive?
• What method can be used to sterilize media that cannot be heated?
• What does a bile esculin positive result indicate?
• Why is it critical to record temperatures regularly?
• "TRIC" conjunctivitis includes which of the following?
• What do the optochin and bacitracin tests have in common?
• Which method is advised for maintaining safety in laboratories regarding equipment?
• What term describes a treatment or process that makes a medical device safe to handle?
• A gram-negative, fastidious bacillus that has been isolated from air-conditioning towers is
• How does one measure the zone of growth inhibition for the Kirby-Bauer susceptibility test?
• How can Mycobacterium tuberculosis be differentiated from Mycobacterium bovis?
• In laboratory settings, what does a positive result in the latex agglutination test indicate?
• Which of the following anaerobes is known to cause antimicrobial-associated diarrhea?
• Hippurate hydrolysis is primarily used to differentiate which organisms?
• Which specimen is usually used for the diagnosis of Hansen's disease?
• After inoculating the Mueller-Hinton plates for the Kirby-Bauer susceptibility test, how long should the plates dry before adding the disk?
• Nonfermenters produce what reaction in triple sugar iron agar (TSIA)?
• What does the Elek test detect?
• Which genera of bacteria are known for their ability to form spores?
• Which organism is considered part of the normal flora of the skin?
• Which organism is linked to ulcerative chancroid?
• Which of the following is a feature of Clostridium difficile?
• What can be said about acid-fast rods in a specimen from nasal mucosa?
• Which type of plasma is employed in the tube coagulase test?
• Spore-forming cells in bamboo pole arrangement of Bacillus anthracis can be found in which of the following?
• Which of the following is a characteristic of alpha-hemolytic streptococci?
• Which of the following organisms is NOT associated with venereal disease?
• Which type of antibiotic is usually preferred for treating infections caused by specific pathogens?
• What is the mordant used in the Ziehl-Neelsen acid-fast stain?
• What term is used to describe dark staining granules in bacteria?
• Which biochemical test is used to identify Group A, beta-hemolytic streptococci?
• What does a positive Quellung test indicate?
• What type of slant and butt reaction do nonfermenters show in TSIA?
• What enzyme removes the amino group from an amino acid?
• The diagnosis of gonorrhoea in males can be made from which of the following?
• What organism is responsible for causing "lumpy jaw"?
• The terms "palisading," "picket fence," and "Chinese letters" describe the common arrangement of which bacteria?
• What kind of media is used for enriching Enterobacteriaceae species?
• Which of the following organisms is known to have no cell wall?
• Refrigeration of the specimen for several months may enhance isolation of which organism?
• What is the main function of bile salts in Salmonella-Shigella agar?
• What color do colonies appear on Brilliant Green Agar?
• Potassium tellurite medium produces what color colonies of Corynebacterium diphtheriae?
• Which of the following is NOT gram-negative?
• If there is too much moisture on the surface of a Mueller-Hinton plate when performing a Kirby-Bauer susceptibility test, what will happen to the growth?
• What reagent is primarily used in the String test?
• In a Tween 80 hydrolysis test, a positive result is indicated by what color change?
• Which of the following are considered diphtheroids?
• Which hemolysin, produced by beta-hemolytic streptococci, is oxygen stable and non-antigenic?
• Recording the temperature of thermometers should be done how often?
• What distinguishes mycoplasmas from true bacteria?
• Which of the following species of Bordetella are urease and oxidase-positive?
• The preferred method of differentiating Staphylococcus aureus from Staphylococcus epidermidis is?
• Which of the following gram-positive cocci are known to ferment glucose?
• Infections with which microorganism may result in the presence of "clue cells"?
• Which species is known for being nitrate reduction positive and niacin positive?
• Which test for bacterial antigen from cerebrospinal fluid is known to be more sensitive and faster?
• Todd-Hewitt broth is primarily recommended for what purpose?
• An organism isolated from a blood culture is a Gram-negative rod, urease and PD positive, with motility that is either positive or negative. Which organism does this describe?
• Which agar is best for isolating a gram-negative, slender, curved rod that causes enteritis?
• Which method is recognized as the most effective for sterilization?
• If a patient is taking antimicrobials, which of the following can neutralize the antimicrobials?
• What is the best medium for isolating Legionella?
• Which of the following organisms produces no haze in a broth culture?
• Edwardsiella tarda is characterized as:
• What is the method of action for beta-lactam antibiotics?
• In which phase of bacterial growth does growth cease due to nutrient exhaustion?
• What color indicates a negative result in the malonate utilization test?
• What sugar is used in Salmonella-Shigella agar?
• In the fractional sterilization process, what sequence correctly describes the steps involved?
• What does gelatinase break down gelatin into?
• Which mycobacteria belong to Group IV (rapid growers)?
• What type of organisms can be identified using the beta-lactamase test?
• What is a common effect of antibiotic resistance in bacterial populations?
• Which biochemical test is used to confirm the presence of Enterococcus?
• Quaternary ammonium disinfectants are known to be easily inactivated by which of the following?
• Which chemical compound is known for its ability to provide effective sterilization when used in appropriate concentration?
• Which of the following organisms cause whooping cough?
• Which of the following is a characteristic of Rickettsia?
• Which test differentiates between slow and rapid growers among mycobacteria?
• IMViC is a series of which of the following tests?
• How is a smear for an acid-fast stain fixed?
• What is the primary purpose of the acid-fast stain?
• The lowest concentration of drug that will kill all but the minimum defined proportion of viable organisms after incubation for a fixed time under a given set of conditions is the definition of the:
• What finding would suggest a significant bacterial infection in cerebrospinal fluid analysis?
• What type of microorganism is Campylobacter categorized as?
• What is the function of CAMP factor produced by group B beta-hemolytic streptococci?
• A gram-negative, anaerobic coccus that produces red fluorescence under ultraviolet light is?
• What type of bacteria does sodium polyanethol sulfonate prevent from affecting blood cultures?
• Why is potassium tellurite used in culture media?
• Which organism is associated with Scarlet fever?
• Which type of bacteria require oxygen-containing compounds to obtain oxygen?
• What is the recommended interval for recording the temperature in freezers?
• Why is an extra tube required when testing three amino acids for decarboxylase?
• What does a slimy colony on blood agar medium typically indicate?
• How important is it to record incubator temperatures for laboratory safety?
• Which of the following is not caused by Rickettsia?
• What indicates vaginal or urethral contamination of urine?
• What inhibitor is used in MacConkey medium?
• Which of the following is not classified as a disinfectant?
• Neisseria meningitides degrades which of the following sugars?
• What is erythema chronicum migrans (ECM)?
• Which method is most sensitive for diagnosing infections with Legionella pneumophila?
• Which of the following organisms is known to be oxidase-positive?
• Which mycobacterium species can reduce nitrates?
• Which of the following describes the function of a disinfectant?
• What is the likely reason for no growth occurring in a bronchial washing despite organisms being seen on a Gram stain?
• Which amino acids are used in the test for decarboxylase?
• What characteristic is most definitive for identifying Staphylococcus aureus?
• What frequency is recommended for general checks of laboratory equipment?
• What interval is ideal for checking water baths in a laboratory setting?
• How do antibiotics typically inhibit bacterial growth?
• In the Ziehl-Neelsen staining method, what is the decolorizer?
• What type of spores are produced by Clostridium tetani?
• What is the significance of a color change in the chromogenic cephalosporin test?
• What color do gram-positive bacteria stain?
• An oxidase-positive, gram-negative coccus from a throat culture may be?
• Which of the following organisms is oxidase-positive?
• What distinguishes the Fite-Faraco acid-fast stain from other acid-fast stains?
• Which type of culture environment is required for the growth of Mycoplasma pneumoniae?
• What is the medium of choice for culturing gonococci and meningococci?
• What type of infection is associated with Mycoplasma pneumoniae?
• Substances, produced by microorganisms, that in very small amounts inhibit other microorganisms are called?
• When should temperatures of incubators and other equipment be recorded?
• What is the role of peptone in Oxidation-Fermentation Medium?
• How frequently should the settings of rpms on the centrifuge be checked?
• In Mueller-Hinton agar, what can cause increased resistance of Pseudomonas aeruginosa to aminoglycosides?
• What methods are utilized for the identification of mycobacteria?
• Which of the following has both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA)?
• What do "PPNG" gonococci produce?
• Which of the following is destroyed by oxygen?
• What is the most common tick-borne disease in the U.S.?
• When performing a bacitracin differentiation test for group A beta-hemolytic streptococci, what is the recommended disk unit?
• Which organism is used for quality control in a dry heat oven?
• The morphological cycle of Rhodococcus equi from coccoid to rod form takes how many hours?
• How often should catalase, oxidase, and coagulase reagents be tested?
• What type of environment does Campylobacter thrive in?
• Which organism is commonly tested for using throat cultures?
• An example of a negative stain is?
• What term describes bacteria that vary in size and shape?
• How many tubes of Oxidation-Fermentation media should be inoculated and what is an important precaution?
• What is the main concern with the overuse of broad-spectrum antibiotics?
• The DNase test is based on which of the following?
• An organism that is both urease and PD negative, with specific TSIA results, is being tested. Which organism is being described?
• The preferred medium for the isolation of Bordetella pertussis is
• Thayer-Martin medium is basically a(an)?
• Which of the following tests can give a presumptive identification of Branhamella catarrhalis?
• What is the primary reason sodium polyanethol sulfonate is used as an anticoagulant in blood cultures?
• A positive tube coagulase test is indicated by what?
• Which organism is most frequently isolated from skin and soft tissue infections?
• Using a sheep blood agar plate helps to eliminate which organism?
• Which type of bacteria are Peptococcus and Peptostreptococcus?
• An organism isolated from a stool culture displays certain reactions, including being motile, LDC positive, and indole-positive. What organism is it likely to be?
• Any organism that is indole-positive and nitrate reduction positive is also likely to be?
• What type of specimen is typically used for the Gram stain procedure in cerebrospinal fluid analysis?
• A Brucella isolate that does not produce H2S, does not require CO2, and is not inhibited by thioinin or basic fuchsin is probably which species of Brucella?
• Which H2S indicator is used in Xylose-Lysine-Deoxycholate agar?
• What is the cause of primary atypical pneumonia?
• What media components are required to demonstrate capsules in bacterial cultures?
• What is the indicator in the methyl red test?
• Why should beta-lactamase tests be performed with growth from primary isolation media?
• Which of the following is a characteristic of Salmonella, Shigella, Escherichia coli, and Serratia?
• What is the indicator in Bismuth-sulfite agar?
• Which characteristic is NOT associated with Morganella morganii?
• Which clostridia produce a double zone of hemolysis around their colonies on blood agar?
• Most Enterobacteriaceae give what type of methyl red and Voges-Proskauer reactions?
• Which colony appearance indicates lactose fermentation on MacConkey medium?
• Chlamydia are cultured in which of the following?
• Non-acid fast bacilli retain which dye?
• What do we call bacteria that can tolerate the presence of oxygen but do not require it for growth?
• Which staphylococcus is most commonly associated with urinary tract infections in young females?
• Which of the following statements is true regarding oxidase tests?
• Resistance to an antibiotic can be transferred from a resistant to a susceptible organism by?
• Why is a first morning urine specimen preferred for urine cultures?
• Which of the following are methods for testing for the production of beta-lactamase?
• An organism isolated from a stool culture is a Gram-negative rod, urease and PD negative, and shows certain results in TSIA. Which organism does this indicate?
• An acid-fast stain that does not use heat as the mordant is which of the following?
• Which bacterial phase is characterized by rapid cell division and growth?
• Which of the following describes Peptococcus and Peptostreptococcus?
• Cultures of Staphylococcus supplies which of the following for cultures of Haemophilus?
• What term describes bacteria that grow in the absence of free oxygen?
• Staphylococcal protein A coated with antiserum is utilized in which serological test for cerebrospinal fluid?
• Which of the following indicates a positive reaction for the beta-lactamase chromogenic cephalosporin method?
• Which of the following is a virulence test for Listeria monocytogenes?
• A relatively slow growing and fastidious, gram-negative rod that produces a characteristic brown pigment on Feeley-Gorman Agar is
• What type of blood is used in blood agar plates for better production of beta-hemolysis of Haemophilus?
• Pneumococci that are resistant to penicillin should be tested for what?
• Smears of cerebrospinal fluid are prepared from which material?
• In the pour-plate method for colony counts, what effect does the amount of agar added have on dilution?
• Propionibacteria are classified as which of the following?
• Which serological test is used as a confirmatory test for streptococci?
• Which of the following differentiates Thayer-Martin medium from modified Thayer-Martin medium?
• What type of organism is Ureaplasma urealyticum?
• What is a significant use of the Antimicrobial Removal Device in microbiology?
• Which microorganism forms colonies that appear embedded in agar, resembling a "fried egg"?
• Identify the purpose of heat in the Ziehl-Neelsen acid-fast staining procedure.
• Acid-fast organisms in tissue are best stained by which of the following methods?
• What is the indicator used in Simmons citrate agar?
• Which factor allows for visibility of acid production by oxidizers in Oxidation-Fermentation Medium?
• What indicates the presence of hydrogen sulfide in triple sugar iron agar (TSIA)?
• A gram-negative, fastidious bacillus that is difficult to stain by the Gram stain is
• What is the characteristic of beta-hemolytic streptococci when grown under certain conditions?
• When doing a susceptibility test with sulfonamides, there are two concentric zones around the disk. How should the zone be measured?
• Which type of toxin is associated with Clostridium difficile?
• What does PRAS stand for in bacteriology?
• Mycobacterial cultures are best incubated in which condition?
• What test is used to identify Mycobacterium species by splitting phenolphthalein?
• Which media enhances pleomorphism and granule production when culturing Corynebacterium diphtheriae?
• What effect do organisms that produce urease have on the medium?
• When should a covered tube of Lowenstein-Jensen medium be observed?
• What does the campy gas mixture primarily consist of?
• Which organism is identified as a slow-growing, unbranched, acid-fast rod that is negative for nitrate reduction?
• A bacitracin-resistant, hippurate hydrolysis positive, bile esculin positive, beta-hemolytic Streptococcus that grows in 6.5% NaCl is probably a(an)?
• Rickettsias are transmitted by which of the following?
• What is the most common pathogen identified in throat cultures?
• What are broad spectrum antibiotics?
• What is the appearance of colonies on Bismuth-sulfite agar?
• A positive urease test for ureaplasmas is indicated by what observation?
• What indicates a positive DNase result after adding 0.1 N HCl to the plate?